The effect of alternating temperature treatment on seed germination of Pulsatilla tongkangensi
DOI:
https://doi.org/10.51258/RJH.2024.22Keywords:
dormancy, full seeds, germination rate, Pasque flower, X-ray imagingAbstract
The effect of alternating temperature on the germination of Pulsatilla tongkangensis seeds was investigated. Open pollinated seeds were wrapped in a moist paper towel for alternating temperature treatments. Seeds were sown in cells filled with Miracle-Gro Potting Mix and then topped with 0.5 cm of Miracle-Gro Seed Starting Potting Mix. The number of germinated seeds was counted daily until no further germination was observed for 3 to 4 days. The maximum germination rate (49%) was achieved in 18 days when seeds were treated at 22°C for 7 days followed by 5 °C for 7 days, and germinated at 20°C. The low germination rate could result from the inclusion of empty seeds. Mature P. tongkangensis seeds may not have a shallow physiological dormancy since germination was not improved with 14 days of 5°C.
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An C.H., Lee H.S., Park Y.I., and Lee J.S. (2010). Habitat survey and seed germination test of Pulsatilla tongkangensis in Du-ta mountain, Sam-cheok City. Proceedings of the Korean Society of Environment and Ecology Conference. 20 (1): 59-61.
Baskin J.M., and Baskin C.C. (2004). A classification system for seed dormancy. Seed Science Research. 14, 1- 16. DOI: https://doi.org/10.1079/SSR2003150
Lee A.K., Yuan T., Suh J.K., Choi D.S., Choi I.Y., Roh M.S., Joung Y.H., Ko J.Y., and Lee J.S. (2010). Pulsatilla tongkangensis, a natural hybrid swarm population hybridized with P. koreana based on RAPD and SNPs of chloroplast DNA. Horticulture Environment and Biotechnology. 51: 409 421. http://www.horticulture.or.kr/english/
Microsoft Corporation. (2019). Microsoft Excel, Available at: https://office.microsoft.com/excel.
Oh H.K., Lee K., Lee Y., and Choung Y. (2014). Geographical distribution and ecology in microhabitats of the rare species, Pulsatilla tongkangensis YN Lee et TC Lee. Korean Journal of Ecology and Environment. 47(spc), 74-82. (In Korean with English abstract). http://dx.doi.org/10.11614/KSL.2014.47(5).074
Sang C.K., Kim E.H., and Kim H.Y. (1993). Germination and life span of Pulsatilla cernua var. koreana seeds. Journal of Korean Society for Horticultural Science. 34:207-212 (In Koran with English Summary).
Sang C-K., Choi B-J., and Koh, J-C.(1996). Effect of storage humidity and temperature on seed germination of Pasque flower (Pulsatilla cernua var. koreana). Journal of Korean Society for Horticultural Science. 37: 47-450 (In Korean with English summary).
USDA Agricultural Research Service National Plant Germplasm System (2024). Germplasm Resources Information Network (GRIN-Taxonomy). National Germplasm Resources Laboratory, Beltsville, MD. Search Taxonomy GRIN-Global (ars-grin.gov). 19 Jan. 2024.
Wang W., and Bartholomew B. (2001). 27. Pulsatilla Miller, Gard. Dict. Abr., ed. 4, (1136). 1754. In Flora of
China 6. 329-333.
Wang F., Zhang L-M, Zhang D., Yu X-m, and Song, H.(2013). Effect of different treatments on seed germination of Pulsatilla turczaninovii and Pulsatilla dahurica. Northern Horticulture. 2: 65-67 (In Chinese with English abstract).
Yoo Y.K., Roh Y.S., Yuan T., and Roh M.S. (2020). Germination of Pulsatilla cernua var. koreana seeds influenced by temperature, light, and GA3 treatments. Acta Horticulturae.129(1): 83-190. DOI:10.17660/ActaHortic.2020.1291.23
Yuan T., Wei Q., and Bauchan G. (2019). Germination of Pulsatilla seeds as influenced by seed morphology, moist 5oC and gibberellin (GA3) treatment, and detection of nickel in seeds. HortScience. 54: 2015- 2023. https://doi.org/10.21273/HORTSCI14056-19
Yuan T., Wei Q., Jourdan P., and Yoo Y.K. (2021). Germination of Pulsatilla cernua var. koreana seeds as influenced by harvest dates and development of seeds analyzed by X-ray imaging
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