ASSESSMENT OF AGRONOMIC PERFORMANCE OF PPV RESISTANT ‘HONEYSWEET’ TRANSGENIC PLUM IN ECOLOGICAL CONDITIONS OF THE NORTHERN ROMANIA
DOI:
https://doi.org/10.51258/RJH.2021.06Keywords:
genetically engineered plum, yield potential, fruit attractivenessAbstract
‘HoneySweet’ is an RNAi based genetically engineered plum resistant to Plum pox virus (PPV). Field trials in different European PPV endemic countries clearly demonstrated that ‘HoneySweet’ plum shows a highly effective and durable resistance to PPV infection. No safety concerns were found by regulatory authorities in the U.S. Expanded studies to assess the agronomic performance of ‘HoneySweet’ plum were performed under PPV endemic and ecological conditions of Northern Romania. An experimental plot including transgenic plum, ‘HoneySweet’, and two conventional plum cultivars, ‘Reine Claude d’Althan’ and ‘Stanley’, was established in spring of 2013. Agronomic performance data collected during 2016-2018 revealed a higher yield potential for transgenic ’HoneySweet’ compared to ‘Reine Claude d’Althan’ and a similarity with ’Stanley’. Average fruit weight highlighted the superior attributes of transgenic ‘HoneySweet’ compared with the two conventional plum cultivars. ‘HoneySweet’ transgenic plum fruits were larger, had lower stone/fruit ratio, and more optimal shape and colour relative to ‘Reine Claude d’Althan’ and ‘Stanley’, and a high level of resistance to PPV infection. These data and observations revealed that ‘HoneySweet’ transgenic plum has good productivity and market potential and is well adapted to PPV endemic and ecological conditions of the Northern Romania.
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References
Atanasoff D. (1932). Plum pox. A new virus disease. Yearbook University of Sofia, Faculty of Agriculture and Silviculture 11, 49-69.
Barba M., Hadidi A., Candresse T. and Cambra M. (2011). Plum pox virus. In Virus-Like Diseases of Pome and Stone Fruits, pp. 185-197.
Bobiş O, Zagrai I., Bonta V., Zagrai L., Mărghitaş L. and Dezmirean D. (2017). Comparative studies on chemical composition of two conventional bred and one genetically engineered plum-fruits. Bulletin of UASMV Cluj, Biotechnology 74(2), 149-156.
Cambra M., Capote N., Myrta A. and Llácer G. (2006). Plum pox virus and the estimated costs associated with sharka disease. Bull OEPP/EPPO Bull. 36,202- 204.
Cociu V. and Oprea St. (1989). Metode de Cercetare in ameliorarea plantelor pomicole. Editura Dacia. Figure 7. Comparative fruit size of ‘Stanley’ (a), ‘Reine Claude d’Althan’ (b) and ‘HoneySweet’ (c) 56
Dosba F., Lansac M. and Eyquaqrd J.F. (1994). Resistance des Prunus a la sharka. EPPO Bulletin 24, 691-696.
Dunez, J. and Sutic, D. (1988). Plum pox virus. In: European Handbook of Plant Diseases. Smith I.M., Dunez J., Eliot R.A., Phillips D.H. andArches S.A. (eds.): pp. 44-46.
Hartmann W. and Petruschke M. (2002). Sharka resistant plums and prunes by utilization of hypersensitivity. Acta Horticulturae 538, 391-399.
Isac M. and Zagrai I. (2006). Current status of Plum pox virus and sharka disease worldwide - Plum pox virus (PPV) in Romania. Bulletin OEPP 36 (2), 213.
Kegler H., Fuchs E., Gruntzig M. and Schwartz S. (1998). Some results of 50 years of research on the resistance to Plum pox virus. Acta Virologica 42, 200-2015.
Labonne G., Yvon M., Quiot J.B., Avinet L. and Llacer G. (1995). Aphids as potential vectors of plum pox virus: comparision of methods of testing and epidemiological consequences. Acta Horticulturae 386, 207-218.
Macovei A. and Diaconu, M. (2001). Viro-geographical data concerning the actual spreading status of the plum pox potyvirus in Romania. Middle Eur. Meet. Plum Pox, Pitesti-Maracineni.
Malinovski T., Cambra M., Capote N., Zawadska B., Gorris T., Scorza R., Ravelonandro M. (2006). Field trials of plum clones transformed with plum pox virus coat protein (PPV CP) gene. Plant disease 90, 1012-1018.
Minoiu N. (1997). Plum Diseases and Pests. In: The Plum, Cociu I., BotuI., Minoiou N., Modoran I. (eds.) Editure Conphys, Pitesti, Romania pp. 343-374.
Nemeth M. (1994). History and importance of plum pov in stone-fruit production. Bull. OEPP 24(3), 525-536.
Neumüller M., Treutter D. and Hartmann W. (2010). Breeding for sharka resistance and high fruit quality in European plum (Prunus domestica l.) at Weihenstephan: breeding strategy and selection tools. Acta Horticulturae 874, 221-228.
Polák J. and Jarošová J. (2012). Susceptibility of plum trees cv. ‘Jojo’ to a Czech isolate of Plum pox virus strain D. Canadian Journal of Plant Pathology 34(2), 283-287.
Polák J., Kundu J., Krška B., Beoni E., Komínek P., Pívalova J. and Jarošová J. (2017). Transgenic plum Prunus domestica L. clone C5 (cv. HoneySweet) for protection against sharka disease. J. Integr. Agric. 16, 516–522.
Ravelonandro M., Scorza R., Bachelier J.C., Labonne G., Levy L., Damsteegt V., Callahan A.M. and Dunez J. (1997). Resistance of transgenic Prunus domestica to Plum pox virus infection. Plant Disease 81, 1231–1235.
Ravelonandro M., Scorza R. and Hammond RW. (2011). Biotechnological aproaches for resistance to viruses, viroids and phytoplasmas. In Virus-Like Diseases of Pome and Stone Fruits, pp. 395-399.
Roy A.S. and Smith I.M. (1994). Plum pox situation in Europe. Bulletin OEPP/EPPO 24, 515-523.
Scorza R., Hily J.M., Callahan Ann, Malinovki T. Cambra M., Capote Nieves, Zagrai I., Damsteegt V., Briard P. and Ravelonandro M. (2007). Deregulation of Plum Pox resistant transgenic plum 'HoneySweet'. Acta Horticulturae 738, 669-674.
Scorza R., Callahan A., Dardick C., Ravelonandro M., Polak J., Malinovski T., Zagrai I., Cambra M., Kamenova V. (2013). Genetic engineering of Plum pox virus resistance – ‘HoneySweet’ plum - from concept to product. Plant Cell, Tissue and Organ Culture. 115: 1-12.
Scorza R., Ravelonandro M., Callahan A., Zagrai I., Polak J., Malinowski T., Cambra M., Levy L., Damsteeght Z., Krska B., Cordts J., Gonsalves D. and Dardick C. (2016). HoneeSweet (C5), the first genetically engineered Plum pox virus – resistant plum (Prunus domestica L.) cultivar. HortScience 51 (5), 601- 603.
Toma S., Isac M., Balan V. and Ivascu A. (1998). Detection of Plum pox virus by enzyme-linked immunosorbent assay in some apricot and peach cultivars and hybrids in Romania. Acta Virologica 42(4), 276-277.
Zagrai I., Zagrai L., Kelemen B., Petricele I., Pamfil D., Popescu O., Preda S. and Briciu A. (2010a). Typing and distribution of Plum pox virus isolates in Romania. Julius-Kuhn-Archiv 427, 342-346.
Zagrai I., Zagrai L., Preda S., Isac M. and Cardei E. (2010b). Incidence of Plum pox virus in Romanian plum orchards. Bulletin UASVM, Horticulture 67(1-2), 488.
Zagrai I., Ravelonandro M., Zagrai L., Scorza R., and Minoiu N. (2011). Overview of the investigations of transgenic plums in Romania. Acta Horticulturae 899, 153–158.
Zagrai L. and Zagrai I. (2020). Further evidence of durable resistance in 'HoneySweet' transgenic plum under natural infection with D and Rec strains of Plum pox virus. Scientific Papers. Series B, Horticulture LXIV (1), 204-209.
Zagrai I., Zagrai L.A., Ravelonandro M., Scorza R., Jakab Z. and Guzu G. (2020). Assessment on the potential decrease of insecticide treatments against aphids in 'HoneySweet' plum (Prunus domestica (L.)) plantings. Acta Horticulturae 1269, 185-190. https://doi.org/10.17660/ActaHortic.2020.1269.25
European and Mediterranean Plant Protection Organization. EPPO. (2021). https://gd.eppo.int/taxon/PPV000/distribution.
Food and Agricultural Organization. FAOSTAT. (2019). www.fao.org/faostat/en/#data/QCL
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