Identification of Arisaema Species by leaf arrangement and molecular markers
DOI:
https://doi.org/10.51258/RJH.2022.01Keywords:
germplasm collection, leaf morphology, molecular analysis, new ornamental plant, sequence analysisAbstract
The objectives of this study were to identify three Arisaema amurense, A. ringens and A. triphyllum specious based on molecular markers and to select accessions with unique morphological characters. Tentative identification based on leaf morphology was verified using single nucleotide polymorphisms (SNPs) generated from sequence analysis of the nuclear transcribed spacer of rDNA gene (nrITS) and the chloroplast intergenic spacer of trnL-trnF gene (cpIS). Specific SNPs from nrITS for A. amurense and A. triphyllum were identified. However, A. ringensspecific SNPs could not be identified because of multiple variations in the nrITS sequences of the species. Species-specific SNPs for the conserved cpIS sequenced in the same species were identified for each species. Morphological characteristics based on the number of leaflets were not sufficient to characterize closely related species such as A. amurense and A. ringens. Arisaema amurense with a unique leaf morphology with variegated, serrated, and denticulate foliage could be selected for further evaluation as a source of new ornamental germplasm.
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References
Gusman G. and Gusman L. (2006). The Genus Arisaema: A monograph for botanists and nature lovers, 2nd ed. Ruggell, Liechtenstein: A. R. G. Gartner Verlag KG.
Joung Y.H., and Roh M.S. (2005). Mapping characterization of Pinus sylvestris var. sylvestriformis based on chloroplast DNA microsatellite markers. Journal of Forest Genetics. 12: 89-98. https://doi.org/10.1007/s11676-013-0325-1
Joung Y.H., Suh J.K., Lee N.S., Eum S.M., Choi I-Y. and Roh M.S. (2010). Identification of Hydrangeaceae accessions of wild origin from Jeju, Korea, using molecular markers. Plant Genetic Resources: 8, 235-241. DOI: https://doi.org/10.1017/S1479262110000286
Ko S.C., and Kim Y.S. (1985). A taxonomic study on genus Arisaema in Korea. Korean Journal of Plant Taxonomy. 15: 67-109.
Lee A.K., Wu X.W., Suh J.K., Joung Y.H. and Riu K.Z., (2013). Screening of genes to identify Arisaema (Araceae) accessions based on single nucleotide polymorphism. Acta Horticulturae. 1000: 475-479. DOI: 10.17660/ActaHortic.2013.1000.67
Lee A.K., Joung Y.H., Wu X.W., Jung H.K., Burk J.C., Sanders L.L. and Suh J.K. (2011). Confirmation of hybrid origin in Arisaema (Araceae) using molecular markers. Scientia Horticulturae. 129: 812-817. https://doi.org/10.1016/j.scienta.2011.03.037
Park S.H., Burchi G., Roh M.S. and Joung Y.H. (2014). Characterization of Kolkwitzia amabilis accessions based on flowering and molecular markers. Scientia Horticulturae. 165: 190-195. https://doi.org/10.1016/j.scienta.2013.11.003
Park S.Y., Joung Y.H., Suh J.K. and Roh M.S. (2022). Evaluation of Hemerocallis germplasm using single nucleotide polymorphisms of nrITS and chloroplast interspacer. Advances in Horticultural Science. 36: 247251 DOI: 10.36253/ahsc-12347
Suh J.K., Lee A.K., Roh M.S., Lee J.S., Lee, J.-S. and Joung Y.H. (2013). Relationships of Lilium species native to Korea analzed by internal transcribed spacers of rRNA. Acta Horticulturae. 1000: 559-565 DOI: 10.17660/ActaHortic.2013.1000.80
Renner S.S. Zhang L.B. and Murata J. (2004). A chloroplast phylogeny of Arisaema (Araceae) illustrates Tertiary floristic links between Asia, North America, and East Africa. American Journal of Botany. 91: 881-888. DOI: 10.3732/ajb.91.6.881
Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular and Biology Evolution. 30: 2725-2729. DOI: 10.1093/molbev/mst197
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