Effect of different types of the medium on micropropagation of Ixora coccinea L.

Authors

  • R.A.H. Onsa Department of Horticulture, Faculty of Agriculture, University of Al zaiem Al azhari, Khartoum, Sudan Author
  • I. Ae. Abdellatif Department of Horticulture Sciences, Faculty of Agriculture Sciences, University of Gezira, Sudan Author
  • M. Ge. Osman Department of Plant Tissue Culture, National Center of Research, Khartoum Sudan Author

DOI:

https://doi.org/10.51258/RJH.2022.06

Keywords:

growth regulator; acclimatization; in vitro; genetic fidelity; DNA

Abstract

Ixora coccinea is one of the important ornamental plants, commercially propagated by stem cuttings. This study aims to determine the effects of different media on micropropagation and rooting and to investigate the genetic fidelity of regenerated plants using the Inter Simple Sequence Repeat (ISSR) marker. For this purpose, Murashige and Skoog (MS), and Woody Plant Medium (WPM) media were used for micropropagation and rooting experiments. All media were supplemented by 5.5g /l gelling agent (Agar) for micropropagation, MS media with different level of sucrose (15,30 ,45 and 60 g/l), 2.0 mg/l BAP with different concentrations of IBA (0.0, 0.1, 0.25 and 0.5 mg/l), for rooting 10mg /l NAA used. In vitro rooted plantlets were transferred to plastic pots containing; peat moss with vermiculite (1:1; 2:1, and 1:2 v/ v) for acclimatization to ex vitro conditions. The ISSR markers were used for the assessment of genetic fidelity between the mother plant and acclimatized plantlets ISSR marker used. Total DNA was extracted from leaves of the acclimated plant by the cetyltrimethyl ammonium bromide method (CTAB), and the data obtained were analyzed and used to generate a similarity matrix using Jaccard’s similarity coefficient. The best result in terms of regeneration of shoots was obtained from 30 g/ l sucrose on a full MS medium. MS media during the incubation period gave the best results in terms of vegetative growth parameters compared to WPM. The estimation of the genetic similarity coefficient based on the ISSR band indicated that acclimatized plantlets derived from the 6th subculture were 100% similar to the mother plant. 

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References

Amin M.N., Ahmed S., Alam M.R and Azad M.A. (2002). In vitro rapid clonal propagation of an ornamental plant Ixora fulgens. Journal of biological sciences, vol. 2:485-488.

Bairu M.W., Fennell C.W. and Staden J. (2006). The effect of plant growth regulators on somaclonal variation in Cavendish Banana (Musa AAA Cv.‘Zelig’). Scientia Horticulturae, vol. 108: 347-351.

Doyle J.J. and Doyle J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull.vol. 19: 11-15.

El Amin H. M. (1990). Ixora- in Trees and shrubs of Sudan. Food and Agricultural Organization (FAO) press. 491pp.

Gamborg O. L, Murashige T. Thorpe T.A. and Vasil I.K. (1976). Plant tissue culture media. In vitro, vol.12:473-78.

George E.F. (1993). Plant propagation by tissue culture. Part1-The technology. Edington: Exegetics limited, pp.337-356.

Hartmann C., Henry Y., Buyser J., Aubry C. and Rode A. (1989). Identification of New Mitochondrial Genome Organizations in Wheat Plants Regenerated from Somatic Tissue Cultures. Theoretical and Applied Genetics, vol.77: 169- 175.

Hartmann H.T., Kester D.E., Davies F.T. and Geneve R.L. (2007). Plant propagation: principles and practices. 6thEdition. Chapter 18. Techniques of in vitro culture for micropropagation and biotechnology. Prentice-Hall, Upper Saddle River. p. 590-624.

Idowu T.O, Ogundaini A.O., Salau A.O., Obuotor E.M., Bezabih M. and Abegaz B.M. (2010). Doubly linked, a-type proanthocyanidin trimer and other constituents of Ixora coccinea leaves and their antioxidant and antibacterial properties. Phytochemistry; 71:2092-2098

Jaccard P. (1908). New researches on the floral distribution. Bull Soc Vaudoise Sci Natl 44: 223–270

Karhu S. T. (1997). Auxalary shoot proliferation of blue Honeysuckle. Plant cell, Tissue and organ culture.vol.48:195-201.

Kim H.S. (2011). Physical properties of the horticultural substrate according to mixing ratio of peat moss, perlite and vermiculite. Korean Journal of Soil Science and Fertilizer 44(3) 321-330.

Lakshmanan P., Lee C.L. and Goh C.J. (1997). An efficient in vitro method for mass propagation of a woody ornamental Ixora coccinea. Plant Cell Rep.vol.16: 572-577.

Li T., Cai H., Wang T., Fu Y., Yang W., Zhao A., Cui Z. and Wang, J. (2019). Plant regeneration in Ixora chinensis from young leaves. Plant Cell, Tissue and Organ Culture, 139(3), 605-608.

Lloyd G., and McCown B. (1980). Commercially Feasible Micropropagation of Mountain laurel ,Kalmia latifolia by Use of Shoot Tip Culture. The International Plant Propagators society vol 30,421-427

Murashige T. and F. Skoog. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant, vol.15: 473–97.

NISIR, National Institute of Science Communication and Information Resources. (2002). The wealth of India, dictionary of Indian raw materials and industrial products—raw materials. New Delhi.

Onsa R.A.H., Abdellatif I.A., Osman, M.G. and Abdullah T.L. (2018). Comparison between uses of growth regulators and natural adjuvant on in vitro micropropagation of Ixora coccinea L. International journal of science, environment and technology .7(5),1794-1801.

Onsa R.A.H., Abdellatif I.A., Osman M.G. and Abdullah T.L. (2018). Effect of Growth Regulators in in vitro micropropagation of Ixora coccinea L. International Journal of Scientific and Research Publications. 8(11),144-149

Pierik R.LM. (1997). In vitro Cultures of higher plants. Dordrecht, the Netherlands: Kluwer academic Publishers, p 21-125.

Raina S.N, Rani V, Kojima T, Ogihara Y, Singh K.P. and Devarumath R.M. (2001) RAPD and ISSR fingerprints as useful genetic markers foranalysis of genetic diversity, varietals identification, and phylogeneticrelationships in peanut (Arachis hypogaea) cultivars and wild species. Genome 44:763–772

Rodrigues P., Tulmann A., Cassieri P. and Mendes B. (1998). Influence of the Number of Subcultures on Somaclonal Variation in Micropropagated Nanicao (Musa Spp.) Acta Horticulturae. vol.490: 469-473.

Rajaseger G. H. T. W., Tan H. T. W., Turner I. M. and Kumar, P. P. (1997). Analysis of Genetic Diversity among Ixora Cultivars (Rubiaceae) using Random Amplified Polymorphic DNA. Annals of Botany, 80(3), 355

Ska S.S., Habib A.M., El shamy M.A. and Soliman H.B. (2010). Micropropagation of Ixora andulata by tissue culture. Journal of horticulture science and ornamental plant, vol.2:111-117.

Smikal P., Valledor L., Rodriguez R. and Griga M. (2007). Assessment of genetic and epigenetic stability in long-term in vitro shoot culture of Pea (Pisum Sativum L.). Plant Cell Reports. 26: 1985-1998.

Thakur P.C. and Kumar H. (2014). In vitro morphogenic response of Ixora parviflora. IJ EK. vol. 1 :4-7.

WCSP (2017). World Checklist of Selected Plant Families. facilitated by the royal botanical garden, kew http://wcsp.scince.kew.org/Retrieved.

Williams E. G. and Maheswaran G. (1986). Somatic embryogenesis:Factors influencing coordinated behavior of cells as an embryogenic group. Annals of Botany vol.57(4): 443-462.

Zeitkiewicz E, Rafalski A, and Labuda D (1994) Genome finger printing by simple sequence repeat (SSR)- anchored polymerase chain reaction amplification. Genomic 20:176–183

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Published

2022-12-15

How to Cite

(1)
R.A.H. Onsa; I. Ae. Abdellatif; M. Ge. Osman. Effect of Different Types of the Medium on Micropropagation of Ixora Coccinea L. RJH 2022, 3, 45-52. https://doi.org/10.51258/RJH.2022.06.

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