Low-Cost Gelling Agent in Tissue Culture Technology for the Orchid Regeneration

Authors

  • P.U. KUMARA General Sir John Kotelawala Defence University, Kandawala Road, Ratmalana, Sri Lanka Author
  • A.G.K.K.M.W. Atapattu General Sir John Kotelawala Defence University, Kandawala Road, Ratmalana, Sri Lanka Author
  • H.M.I. Herath Royal Botanical Garden, Peradeniya, Sri Lanka Author
  • D.L.C.K. Fonseka Faculty of Agriculture, University of Ruhuna, Mapalana, Sri Lanka Author
  • K.K.I.U. Aruna Kumara Faculty of Agriculture, University of Ruhuna, Mapalana, Sri Lanka Author
  • F.M.M.T. Marikar General Sir John Kotelawala Defence University, Kandawala Road, Ratmalana, Sri Lanka Author

DOI:

https://doi.org/10.51258/RJH.2022.07

Keywords:

alternative gelling agent, alternative water source, Orchid subculture

Abstract

Orchid is one of the most important cut flowers, grown in Sri Lanka for export and local market. One of the majors constrains in expanding the orchid cultivation is limited supply of quality planting materials. Micropropagation techniques can be successfully applied for mass propagation of orchids by the micropropagation is expensive thus increase the cost of production. Therefore, this research was undertaken for replacement of expensive agar for in vitro subculturing of Cattaleya seedlings. Two-month-old Cattleya seedlings were subcultures on Murashige and Skoog media (MS) as basal media. As gelling agents’ sago, semolina, corn flour, semolina + agar, and corn flour + agar was used. Control treatment was carried out with agar. Cultures were maintained inside the culture room. Number of leaves, number of roots, leaf length, leaf width and percentage of contaminations were recorded at four-week intervals. Sago and semolina + agar showed higher performance and low contamination percentage as alternative gelling agent in the MS media. The results of this study propose that sago, semolina, and corn flour can be used as an alternative gelling agent in MS media for subculture practices of orchids. 

Downloads

Download data is not yet available.

References

Ahloowalia B.S., Prakash J., Savangikar V.A. and Savangikar C. (2004). Plant tissue culture. Low cost options for tissue culture technology in developing countries. International Atomic Energy Agency, Vienna, pp.3-11.

Álvarez S.P., Tapia M.A.M., Vega M.E.G., Ardisana E.F.H., Medina J.A.C., Zamora G.L.F. and Bustamante D.V. (2019). Nanotechnology and plant Tissue Culture. In Plant Nanobionics (pp. 333-370). Springer, Cham.

Arditti J. and Ernst R. (1993). Micropropagation of orchids. John Wiley & Sons, New York, USA.

Bose C., Guo J., Zimniak L., Srivastava S.K., Singh S.P., Zimniak P. and Singh S.V. (2002). Critical role of allyl groups and disulfide chain in induction of Pi class glutathione transferase in mouse tissues in vivo by diallyl disulfide, a naturally occurring chemopreventive agent in garlic. Carcinogenesis, 23(10), pp.1661-1665.

Boxus P. and Druart P. (1986). Virus-free trees through tissue culture. In Trees I (pp. 24-30). Springer, Berlin, Heidelberg.

Debergh P.C. (1983). Effects of agar brand and concentration on the tissue culture medium. Physiologia plantarum, 59(2), pp.270-276.

George P. and Manuel J. (2013). Low cost tissue culture technology for the regeneration of some economically important plants for developing countries. International Journal of Agriculture, Environment and Biotechnology, 6(Special Issue), pp.703-711.

Kodym A., Afza R., Forster,B.P., Ukai Y., Nakagawa H. and Mba C. (2012). Methodology for physical and chemical mutagenic treatments. Plant mutation breeding and biotechnology, pp.169-180.

Kodym A. and Zapata-Arias F.J. (2001). Low-cost alternatives for the micropropagation of banana. Plant Cell, Tissue and Organ Culture, 66(1), pp.67-71.

Mohapatra P.P. and Batra V.K. (2017). Tissue culture of potato (Solanum tuberosum L.): A review. International Journal of Current Microbiology and Applied Sciences, 6(4), pp.489-495.

Murdad R., Hwa K.S., Seng C.K., Latip M.A., Aziz Z.A. and Ripin R. (2006). High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocorms technique. Scientia Horticulturae, 111(1), pp.73-79.

Norhayati Y., NorAini M.F., Misri K., Marziah M. and Azman J. (2011). α-tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissues and callus cultures. Pertanika J. Trop. Agric. Sci, 34(2), pp.331-339.

Ogero K.O., Mburugu G.N., Mwangi M., Ngugi M.M. and Ombori, O. (2012). Low cost tissue culture technology in the regeneration of sweet potato (Ipomoea batatas (L) Lam).

Pierik and R.L.M. (1991). Commercial aspects of micropropagation. In Horticulture—new technologies and applications (pp. 141-153). Springer, Dordrecht.

Prakash S., Hoque M.I. and Brinks T. (2004). Culture media and containers. Low cost options for tissue culture technology in developing countries, FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, pp.29-40.

PrasadK. (2019). A new species of Habenaria (Orchidaceae) from the Western Ghats, India. Webbia, 74(1), pp.63-66.

Tokuhara K. and Mii M. (2001). Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae). In Vitro Cellular & Developmental Biology-Plant, 37(4), pp.457-461

Downloads

Published

2022-12-15

How to Cite

(1)
P.U. KUMARA; A.G.K.K.M.W. Atapattu; H.M.I. Herath; D.L.C.K. Fonseka; K.K.I.U. Aruna Kumara; F.M.M.T. Marikar. Low-Cost Gelling Agent in Tissue Culture Technology for the Orchid Regeneration. RJH 2022, 3, 53-60. https://doi.org/10.51258/RJH.2022.07.

Similar Articles

1-10 of 18

You may also start an advanced similarity search for this article.