Assessment of the technological performance of some Saccharomyces and non-Saccharomyces indigenous yeast strains
DOI:
https://doi.org/10.51258/RJH.2022.22Keywords:
yeast, killer factor, volatile compounds, sequential fermentation, starter cultures.Abstract
The study aimed to assess the technological potential of four indigenous Saccharomyces cerevisiae strains (S. cer. 4.1.11, S. cer. 4.3, S. cer. 4.6 and S. cer. 4.10), as possible sources for starter cultures. The experiments were carried out at micropilot level on the natural must of 'Fetească albă' cultivar. The evaluation of the yeasts was carried out according to the chemical parameters and volatile compounds analysed in the obtained wines compared to the wine obtained with a commercial starter culture (CSC). The values of the main physico-chemical parameters analyzed in the obtained wines were similar to those determined in the control wine. The average values of the volatile compounds with positive impact on the wine aroma ranged within the interval 177.46 - 217.81 mg/L, a higher value compared to the control wine, respectively 166.33 mg/L. The use of the indigenous strain Torulospora delbrueckii (T.d 10) in association with the strains S. cer. 4.1.11 or S. cer. 4.10 led to an increase of 12.56%, respectively 8.30% in glycerol concentrations, as well as an increase of 11.94% to 14.49% in the average concentration of volatile compounds. Harnessing the oenological potential of the yeasts tested in sequential fermentations proved dependent on the time allowed for the development of the strain T.d 10, namely 24 and 48 hours, as well as on the yeasts used. Thus, in the wines obtained by the association T.d 10/ S. cer. 4.10, in which the development of the culture T.d 10 was carried out for 48 hours, we noticed an increase of 12.52% and, respectively 32.95%, in the average of volatile compounds, compared to the monoculture wine for the same S. cer. 4.10 strain and to the control wine (CSC).
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References
Arslan E., Çelik Z.D. and Cabaroglu T. (2018). Effects of pure and mixed autochthonous Torulaspora delbrueckii and Saccharomyces cerevisiae on fermentation and volatile compounds of Narince wines. Foods 7(9): 147. doi: 10.3390/foods7090147.
Bely M., Stoeckle P., Masneuf-Pomarede I. and Dubourdieu D. (2008). Impact of mixed Torulaspora delbrueckii- Saccharomyces cerevisiae culture on high sugar fermentation. Int. J. Food Microbiol. 122: 312-320.
Benito Á., Calderón F. and Benito S. (2019). The influence of non-Saccharomyces species on wine fermentation quality parameters. Fermentation 5(3):54. https://doi.org/10.3390/fermentation5030054.
Benito S. (2018). The impact of Torulaspora delbrueckii yeast in winemaking. Appl. Microbiol. Biotechnol. 102(7): 3081-3094.
Brîndușe E., Nechita A., Ion M., Pașa R., Fîciu L. and Ciubucă A. (2022). Valorificarea biodiversității drojdiilor autohtone de vinificație în scopul obținerii vinurilor cu tipicitate de areal viticol (Iași, Romania: PIM). pp. 19-27.
Comitini F., Gobbi M., Domizio P., Romani C., Lencioni L., Mannazzu I. and Ciani M. (2011). Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiol. 28: 873-882.
Dabhole M. and Joishy K. (2005). Production and effect of killer toxin by Saccharomyces cerevisiae and Pichia kluyveri on sensitive yeasts and fungal pathogens. Indian J. Biotechnol. 4: 290-292.
Dashko S., Zhou N., Tinta T., Sivilotti P., Lemut M.S., Trost K., Gamero A., Boekhou, T., Butinar L., Vrhovsek U. and Piskur J. (2015). Use of non-conventional yeast improves the wine aroma profile of Ribolla Gialla. J. Ind. Microbiol. Biotechnol. 42: 997-1010.
Kontogiannatos D., Troianou V., Dimopoulou M., Hatzopoulos P. and Kotseridis, Y. (2021). Oenological potential of autochthonous Saccharomyces cerevisiae yeast strains from the greek varieties of Agiorgitiko and Moschofilero. Beverages 7 (2): 27. Doi: 10.3390/beverages7020027.
Loira I., Vejarano R., Bañuelos M.A., Morata A., Tesfaye W., Uthurry C., Villa, A., Cintora I. and Suárez-Lepe J.A. (2014). Influence of sequential fermentation with Torulaspora delbrueckii and Saccharomyces cerevisiae on wine quality. LWT Food Sci. Technol. 59: 915-922.
Mecca D., Benito S., Beisert B., Brezina S., Fritsch S., Semmler H. and Rauhut D. (2020). Influence of nutrient supplementation on Torulaspora delbrueckii wine fermentation aroma. Fermentation 6 (1): 35. https://doi.org/10.3390/fermentation6010035.
Nechita A., Filimon V.R., Pașa, R., Damian D., Zaldea G., Filimon R. and Zaiț M. (2020). Oenological characterization of some yeast strains isolated from the Iași vineyard Romania. Romanian J. Hort.1: 14.148.
OIV (2019). Compendium of international methods of wine and must analysis. Vol. I. International Organisation of Vine and Wine, Paris, France.
Padilla B., Zulian, L., Ferreres, À., Pastor, R., Esteve-Zarzoso, B., Beltran, G., and Mas, A. (2017). Sequential inoculation of native non-Saccharomyces and Saccharomyces cerevisiae strains for wine making. Front. Microbiol. 8: 1293. doi: 10.3389/fmicb.2017.01293.
Sadoudi M., Tourdot-Maréchal R., Rousseaux S., Steyer D., Gallardo-Chacón J.J., Ballester J., Vichie S., Guérin-Schneider R., Caixache J. and Alexandre H. (2012). Yeast-yeast interactions revealed by aromatic profile analysis of Sauvignon Blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts. Food Microbiol. 32 (2): 243-253.
Sangorrín M., Zajonskovsky I., van Broock M. and Caballero A. (2002). The use of killer biotyping in an ecological survey of yeast in an old Patagonian winery. World J. Microbiol. Biotechnol. 18: 115-120.
Taillandier P., Lai Q.P., Julien-Ortiz A. and Brandam C. (2014). Interactions between Torulaspora delbrueckii and Saccharomyces cerevisiae in wine fermentation: influence of inoculation and nitrogen content. World. J. Microbiol. Biotechnol. 30 (7): 1959-1967.
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