SELECTION OF AUTOCHTHONOUS SACCHAROMYCES AND NON-SACCHAROMYCES YEASTS STRAINS ACCORDING TO THEIR EXTRACELLULAR ENZIMATIC ACTIVITY
DOI:
https://doi.org/10.51258/RJH.2020.16Keywords:
fermentation, enzymatic activity, selection, Saccharomyces, non-Saccharomyces.Abstract
The aim of the present study was to investigate the production of extracellular enzymes in a number of twenty six autochtonous Saccharomyces and non- Saccharomyces strains selected in Dealu Mare region for wine production. The strains were screened for the production of extracellular b-glucosidase, esterase, pectinase and protease activity by inoculation the yeast strains onto selective media. All Saccharomyces tested strains showed at least two enzymatic activities while non- Saccharomyces strains showed activity at least for one enzyme. The weakest activity was recorded in case of β-glucosidase. Most of the tested strains exhibit more or less intense activity for polygalacturonase/pectinase and protease. This study put into evidence the potential of autochtonous and especially of non-Saccharomyces strains as source of production of secondary compounds which can play an important role in improving the quality of wines.
Downloads
References
Buzzini P., Martini A., (2002). Extracellular enzymatic activity profiles in yeast and yeast-like strains isolated from tropical environments, Journal of Applied Microbiology, 93, 1020–1025.
Charoenchai, C., Fleet, G.H., Henschke, P.A., Todd, B.E.N., (1997), Screening of non-Saccharomyces wineyeasts for the presence of extracellular hydrolytic enzymes. Australian Journal of Grape Wine Research, 3,2 8.
Comitini F., Gobbi M., Domizio P., Romani C., Lencioni L., Mannazzu I., Ciani M., (2011), Selected nonSaccharomyces wine yeasts in controlled multistarter fermentations with Saccharomycescerevisiae, FoodMicrobiology, 28, 873-882.124
Dutraive O., Benito S., Fritsch S., Beisert B., Patz C.-D., Rauhut D., (2019),Efect of Sequential Inoculation with Non-Saccharomyces and Saccharomyces Yeasts on RieslingWine Chemical Composition, Fermentation 2019, 5, 79; doi:10.3390/fermentation 5030079.
Fernanda G., Bruna C. A., Gildo Almeida da Silva, Geraldo P., Tania M. B.,(2015), Autochthonous yeasts with β-glucosidaseactivity increase resveratrol concentration duringthe alcoholic fermentation of Vitis labrusca grape must, Journal of Functional Foods, 19, 288–295.
Fia G., Giovani G., Rosi I., (2005), Study of β-glucosidase production by wine-related yeasts duringalcoholicfermentation. A new rapid fluorimetric method to determine enzymatic activity, J. Appl. Microbiol,, 99,(3), 509–517.
Jolly N.P., Varela C., Pretorius I. S., (2014), Not your ordinary yeast: non-Saccharomyces yeasts in wine production, FEMS Yeast Res., 14, 215–237.
Rosi I., Vinella M., Domizio P., (1994), Characterization of β-glucosidase activity in yeasts of oenological origin. Journal of Applied Microbiology, vol. 77, 519-527.
Slifkin, M., (2000), Tween 80 opacity test responses of various Candida species. Journal of Clinical Microbiology, 38, 4626–4628.
Strauss M.L.A., Jolly N.P., Lambrechts M.G., Rensburg P., (2001), Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts, Journal of Applied Microbiology, 91, 182–90.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 ROMANIAN JOURNAL OF HORTICULTURE

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.